This actin is a major constituent of the contractile apparatus and one of Actins are highly conserved proteins that are involved in cell It is also known as BRWS1 or PS1TP5BP1.ThisĮncodes one of six different actin proteins. The official symbol of beta-actin gene is ACTB (provided by HGNC). (HepG2: human HeLa: human SVT2: mouse A549: human COS7: monkey Jurkat: human MDCK: canine PC12: rat Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-beta-actin monoclonal OriGene offers high quality quality of Anti-beta actin

Beta-Actin Western Blotīeta-actin, is usually used as a loading control for Western Blot to normalize the levels of protein detected byĬonfirming that protein loading is the same across the gel. Beta-actinĪ major constituent of the contractile apparatus. These amino acid differences confer different biochemical properties between the two isoforms. In striated and smooth muscle cells, whereas beta-actin and gamma-actin isoforms are ubiquitously expressed.Ĭonserved from birds to mammals, beta-actin and gamma-actin differ at only four biochemically similar amino acid Actins are highly conserved proteins that are involved in cell motility, structure, and integrity.Īre six different but highly conserved actin isoforms in vertebrates. Protein detected by confirming that protein loading is the same across the gel. They can be used to normalize the levels of Beta-actin Loading Control Beta-Actin Proteinīeta-actin, also known as a 'housekeeping' protein, is usually used as a loadingĬontrol, for among others, the integrity of cells, protein degradation, in PCR and Western blotting.Ĭontrols are essential for proper interpretation of western blots.

Actin western blot size